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Image Search Results
Journal: Nature Communications
Article Title: Entire expressed peripheral blood transcriptome in pediatric severe malarial anemia
doi: 10.1038/s41467-024-48259-4
Figure Lengend Snippet: Validation of the RNA-Seq results was performed by comparing the significant ( p < 0.050) DEGs in the transcriptome analysis with those that were significant in a Qiagen targeted-RNA sequencing panel (491 immune response genes) in a different cohort of Kenyan children [SMA ( n = 21) and non-SMA ( n = 23). a Heatmap illustrating the comparison of significant ( p < 0.050) DEGs between the two datasets. The Y-axis depicts the gene pairs, and the X-axis represents the assay type. The color scale depicts fold regulation (Log 2 ). Statistical significance determined using a generalized linear model with a negative binomial distribution, p < 0.050. b Correlation scatter plot demonstrating the relationship between significantly expressed genes in targeted QIAseq analysis (Log 2 FoldChange, Y-axis) versus transcriptome data (Log 2 FoldChange, X-axis). A strong positive correlation of DEGs using two-tailed Spearman’s test ( r = 0.612; 95% confidence interval, 0.496-0.706; p = 1.842E−16) validates concordance between the two sequencing methods.
Article Snippet: Total RNA (125 ng) was used to synthesize cDNA using the First Strand Kit reagents (Qiagen) specific for the Targeted Human Inflammation and
Techniques: Biomarker Discovery, RNA Sequencing, Comparison, Two Tailed Test, Sequencing
Journal: Nature Communications
Article Title: Entire expressed peripheral blood transcriptome in pediatric severe malarial anemia
doi: 10.1038/s41467-024-48259-4
Figure Lengend Snippet: To assess the alignment between DEGs identified from RNA-Seq and changes in protein levels, whole blood transcriptome data were compared with protein abundance measured in plasma using a 7k SomaScan platform. MetaCore TM facilitated the mapping of genes to their respective protein products for 35 children (n = 19 non-SMA and n = 16 SMA) with both available measurements. The analysis identified 405 gene/protein pairs with a significant association ( p < 0.050). a Heatmap showing the comparison of significant ( p < 0.050) gene/protein pairs between the two datasets. The Y-axis depicts the gene/protein pairs, while the X-axis represents the assay type. The color scale depicts fold regulation (Log 2 ), and p < 0.050 calculated using a generalized linear model with a negative binomial distribution. b Correlation scatter plot demonstrating the relationship between significantly expressed protein targets (Log 2 FoldChange; Y-axis) and genes (Log 2 FoldChange; X-axis). A two-tailed Spearman’s test indicated a modest positive concordance between gene expression and protein abundance ( r = 0.205; 95% confidence interval, 0.107–0.299); p = 3.200E−5].
Article Snippet: Total RNA (125 ng) was used to synthesize cDNA using the First Strand Kit reagents (Qiagen) specific for the Targeted Human Inflammation and
Techniques: RNA Sequencing, Quantitative Proteomics, Clinical Proteomics, Comparison, Two Tailed Test, Gene Expression